Product Overview
The CELLIPOWER Series cell culture bioreactors are classic benchtop systems designed specifically for laboratory-scale cell cultivation. Constructed from high-quality borosilicate glass, these bioreactors offer excellent thermal and chemical resistance, optical clarity for real-time observation, and compatibility with standard autoclaving (121 °C), ensuring a sterile and reliable environment for sensitive biological processes.
Developed by Shanghai Ritai Medicine Equipment Project Co., Ltd., the CELLIPOWER series integrates cutting-edge bioengineering principles with industrial-grade control architecture to deliver a comprehensive solution for modern biopharmaceutical and biotechnological applications. The system supports the cultivation of suspension cells, microcarrier-based adherent cells, and fiber disk-adherent cells, making it ideal for the development and production of therapeutic antibodies, cell and gene therapies, vaccines, stem cells, exosomes, and more.
All models comply with cGMP standards and feature an industrial-grade touchscreen computer paired with a Siemens PLC control system, enabling precise regulation of critical process parameters—including temperature (±0.1 °C), pH (±0.01), dissolved oxygen (DO, ±1%), and agitation speed—through advanced PID algorithms and real-time feedback.
Equipped with a 4-channel peristaltic pump feeding system and thermal mass flow controllers (MFCs) for gas delivery, the platform supports complex feeding strategies such as batch, fed-batch, and perfusion modes, facilitating flexible and scalable process development.
With exceptional reproducibility, operational efficiency, and user-friendly design, the CELLIPOWER series serves as an ideal platform for upstream process development in both academic and industrial settings.
Core Technical Features1. Precision Agitation Systems
Tailored to different cell culture requirements, the series offers three optimized impeller configurations:
CELLIPOWER01 (for suspension cells): Features a propeller-type impeller that delivers efficient axial mixing with low shear stress (20–200 rpm, ±1 rpm accuracy). Ideal for high-density cultures of CHO, HEK293T, and other suspension-adapted lines, it achieves up to 1.5× higher mixing efficiency than conventional impellers while minimizing cell damage.
CELLIPOWER02 (for microcarrier-based adherent cells): Uses a paddle impeller combined with a microporous sparging system to maintain gentle hydrodynamic conditions (shear stress: 0.1–0.3 W/m³). This setup prevents cell aggregation and microcarrier clumping, enhancing viability and productivity for applications like viral vector or vaccine production.
CELLIPOWER03 (for fiber disk-adherent cells): Also employs a paddle impeller but integrates a rotating dispersion sparger that eliminates direct bubble bursting at the liquid surface. This further reduces mechanical stress on sensitive cells such as MSCs and iPSCs, while ensuring uniform oxygen distribution—critical for exosome production and stem cell expansion.
All models use AC stepper motors with PID-controlled speed regulation, supporting dynamic adjustment based on culture phase to minimize manual intervention.
2. High-Precision Sensor Suite
Real-time monitoring and tight control of the culture environment are enabled by industry-leading sensors:
Temperature: Compact digital transmitter with ±0.1 °C accuracy; range: 5–65 °C (above cooling water temp); response rate up to 0.5 °C/min.
pH: Range 0–14, accuracy ±0.01 (significantly better than typical ±0.1 systems); rapid response (<30 sec); controlled via CO₂ and base addition.
Dissolved Oxygen (DO): Range 0–200%, accuracy ±1%; auto-calibrating sensor; regulated by blending O₂, N₂, CO₂, and air.
Liquid Level: Stainless steel probes with ±1 mL precision; supports automated feeding and media exchange.
All sensors feature removable designs for easy calibration or replacement and operate at a sampling frequency of up to 10 Hz for high-fidelity data capture.
3. Intelligent Gas Control System
Precise gas management ensures optimal metabolic conditions:
Mass Flow Controllers (MFCs): Thermal-type MFCs deliver gas flows from 0.1 to 5 SLPM with ±1% accuracy across four gases (O₂, N₂, CO₂, Air).
Gas Dispersion Technologies:
Microporous sparging (CELLIPOWER01/02): Generates bubbles 100–300 μm in diameter to minimize shear.
Rotating dispersion sparging (CELLIPOWER03): Distributes gas evenly without surface bubble collapse.
Sterile Filtration: Inlet (37 mm) and outlet (50 mm) filters made of 0.2 μm PTFE, reusable for >100 cycles.
Exhaust Condenser: 316L stainless steel unit removes moisture with >95% efficiency, preventing backflow contamination.
The gas system dynamically coordinates with pH and DO controls to maintain ideal culture conditions throughout the run.
4. Flexible Feeding System
The integrated 4-channel peristaltic pump system enables versatile nutrient and reagent delivery:
Each pump operates independently at 30 rpm, with duty cycle adjustable from 0–100%.
Feeding accuracy: ±0.5% of setpoint.
Supports batch, fed-batch, and perfusion modes—including continuous medium exchange for high-density cultures.
Four standardized clamp-style ports allow quick connection of glucose, base, feed, or antifoam solutions.
All feeding events are automatically logged (volume and timestamp) with ±1 mL cumulative accuracy and exportable via SCADA software.
5. Distributed Control Architecture
Built for scalability and compliance:
Control Hardware: Industrial touchscreen PC + Siemens PLC.
SCADA Software: Fully compliant with cGMP; supports data logging, trend analysis, audit trails, and electronic signatures.
Data Storage: 128 GB onboard (up to 20 weeks of data); expandable to 1 TB for long-term archiving.
User Management: Up to 23 operator accounts with three-tier permission levels and password protection.
Synchronization: Multi-unit operations show <±0.1% deviation, enabling high-throughput screening.
Connectivity: Supports Modbus/TCP and EtherNet/IP protocols for seamless integration into larger bioprocessing workflows.
This architecture facilitates smooth scale-up from lab to pilot or production, reducing development timelines.
6. Sampling & Monitoring
Designed for sterility and data integrity:
Aseptic Sampling: Glass vials + 3-way valve + clamp seal allow online sampling without interrupting culture.
Sampling Range: 1–50 mL (adjustable); precision ±1 mL; up to once per minute.
Real-Time Monitoring: Tracks temperature, pH, DO, agitation, level, and pressure with graphical display and alarm functions.
Data Export: CSV/Excel formats for offline analysis or regulatory submission.
Process Optimization Tools: Built-in DOE (Design of Experiments) and RSM (Response Surface Methodology) modules accelerate media and process optimization.
Application Areas & Performance Highlights
1. Biopharmaceuticals
Antibody Production: Achieves >1.0 × 1 ⁷ cells/mL with CHO/HEK293T; boosts titer by >30%.
Cell Therapy: >95% viability for T/NK/MSC cultures; low-shear design preserves function.
Vaccines: Supports AAV/lentivirus production with titers up to 1 × 10¹⁰ IU/L.
Stem Cells: Enables 20-fold expansion of iPSCs/MSCs with minimal differentiation.
Exosomes: Yields up to 1 × 10¹¹ particles/mL under tightly controlled pH/DO.
2. Industrial Enzymes
Cellulase: Specific activity up to 1.9 U/mg (Pichia pastoris) at 52 °C, pH 5.5.
Protease: Yields 50 U/mL from Bacillus/lactic acid bacteria.
High-Throughput Screening: 50% faster strain/process evaluation via multi-reactor parallel runs.
3. Probiotics & Wastewater Treatment
Probiotic Expansion: 30% higher yield for Bacillus/nitrifying bacteria.
Algal-Bacterial Symbiosis: Achieves >90% COD removal; 96.6% NH₄⁺-N and 98.6% PO₄³⁻-P removal in bioremediation.
4. Plant Cell Culture
Suspension Cultures: Densities up to 5 × 10⁶ cells/mL.
Immobilized Cultures: >90% cell attachment on microcarriers/fiber disks.
Co-cultivation: 40% increase in secondary metabolite production via plant-microbe synergy.
Key Advantages
| Category |
Advantage |
| Material |
Borosilicate glass: autoclavable (121°C), >95% visual clarity, Ra ≤ 0.6 μm smoothness, reusable for >5 years |
| Precision |
Industry-leading control: Temp ±0.1°C, pH ±0.01, DO ±1% |
| Scalability |
Supports 1–16 parallel units with synchronized operation |
| Usability |
Intuitive GUI, <1-sec system response, modular configuration |
| Compliance |
Full DQ/IQ/OQ/PQ documentation; audit trail; 21 CFR Part 11-ready |
| Efficiency |
Automated feeding, real-time analytics, and DOE tools shorten development cycles |
Technical Specifications
| Category |
Item |
CELLIPOWER01 |
CELLIPOWER02 |
CELLIPOWER03 |
MICROIPOWER07 |
| Tank |
Total Volume |
1L |
3L |
5L |
10L |
15L |
| Working Volume |
0.8L |
2L |
3.5L |
7.5L |
10.5L |
| Aspect Ratio |
1:02 |
01:01.6 |
| Overall dimensions |
H540xW390xD485mm |
⌀210xH690mm |
⌀240xH758mm |
⌀290xH820mm |
⌀330xH910 mm |
| Sterilization dimensions |
⌀106xH270mm |
⌀200xH520mm |
⌀220xH570mm |
⌀260xH630mm |
⌀300xH720 mm |
| Material |
Borosilicate Glass |
| Category |
Item |
CELLIPOWER01 |
CELLIPOWER02 |
CELLIPOWER03 |
MICROIPOWER07 |
| Type |
Growth |
Suspension Cell |
Adherent Cell Microcarrier |
Adherent Cell Fiber Disk |
Microbial Fermentation |
| Ventilation |
Micropore-Dispersed Bubbles Burst On Re-Entry |
Micropore-Dispersed Bubbles Burst On Re-Entry |
Rotary-Dispersed Bubbles Burst On Re-Entry, No Direct Cell Contact. |
Bubbles Dispersed By High-Speed Rotating Impellers Burst On Re-Entry |
| Cell Distribution |
Fully Suspended: No Attachment, Uniform Distribution. |
Microcarriers Suspended, Carrier-Dep Distribution |
Cells Mostly Stationary: Higher Density |
Fully Suspended: No Attachment, Uniform Distribution. |
| Agitation |
Method |
Top Agitation, Mechanically Driven, AC Stepper Motor, PID Control |
| Display |
Digital Display, ±1rpm |
| Speed Range |
20～200rpm |
50～1000rpm |
| Impeller Types |
Propeller |
Paddle |
Paddle |
Flat blade turbine |
| Sensors |
pH |
pH Range: 0~14, PID Control, Accuracy: ±0.01, Connected to Alkali and CO2 |
| DO |
DO Range: 0~200%, PID Control, Accuracy: ±1%, Linked with O2, N2, and Air |
| Liquid Level |
Stainless Steel Probes, PID Control, Using 1~2 Probes |
| Temperature |
Compact Temperature Transmitter, Digital Display Accuracy: ±0.1°C; |
| Gas Control |
Flow Measurement |
Thermal mass flow controller, flow rate 0.1～5 SLPM |
| Gas Control System |
N2, O2, CO2, Air |
O2, Air |
| Filter |
Inlet: 37mm, 0.2μm, PTFE. Outlet: 50mm, 0.2μm, PTFE |
| Exhaust Condenser |
316LStainless Steel |
| Liquid Feeding |
4 Peristaltic Pumps |
Flow Accumulation; Speed: 30rpm; Adjustable Work Time:0～100% |
| Temperature Control |
Method |
PID Regulation, Cooling Water / Electric Heating Blanket, Control Accuracy: ±0.2°C; |
| Control Range |
5°C To 65°C (above cooling water temp) |
| Control System |
1L |
All-In-One PC+ Siemens Small Programmable Logic Controller |
| 3L,5L,10L,15L |
Industrial-Grade Tablet Touch Computer + Siemens PLC. |
| Data Recording |
Computer (PC) Configuration SCADA Software |
Conclusion
The CELLIPOWER Series represents a powerful, versatile, and compliant solution for advanced cell culture process development. By combining borosilicate glass durability, precision environmental control, and intelligent automation, it bridges the gap between research innovation and GMP manufacturing.
Whether you're optimizing a monoclonal antibody process, developing a novel cell therapy, producing viral vectors, or exploring sustainable biomanufacturing with algae or enzymes, the CELLIPOWER platform provides the flexibility, reliability, and data integrity needed to accelerate your journey from lab to clinic—or market.
Choose CELLIPOWER to empower your science with engineering excellence.
